MOL-PCR and xMAP Technology A Multiplex System for Fast Detection of Food- and Waterborne Viruses
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG32__%2F21%3AN0000003" target="_blank" >RIV/60162694:G32__/21:N0000003 - isvavai.cz</a>
Alternative codes found
RIV/00027162:_____/21:N0000078 RIV/62157124:16270/21:43879156 RIV/00216224:14310/21:00122387
Result on the web
<a href="https://doi.org/10.1016/j.jmoldx.2021.03.005" target="_blank" >https://doi.org/10.1016/j.jmoldx.2021.03.005</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jmoldx.2021.03.005" target="_blank" >10.1016/j.jmoldx.2021.03.005</a>
Alternative languages
Result language
angličtina
Original language name
MOL-PCR and xMAP Technology A Multiplex System for Fast Detection of Food- and Waterborne Viruses
Original language description
Viruses are common causes of food- and waterborne diseases worldwide. Conventional identification of these agents is based on cultivation, antigen detection, electron microscopy, or real-time PCR. Because recent technological advancements in detection methods are focused on fast and robust analysis, a rapid multiplexing technology, which can detect a broad spectrum of pathogenic viruses connected to food or water contamination, was utilized. A new semiquantitative magnetic beadebased multiplex system has been designed for simultaneous detection of several targets in one reaction. The system includes adenoviruses 40/41 (AdV), rotavirus A (RVA), norovirus (NoV), hepatitis E virus (HEV), hepatitis A virus (HAV), and a target for external control of the system. To evaluate the detection system, interlaboratory ring tests were performed in four independent laboratories. Analytical specificity of the tool was tested on a cohort of pathogenic agents and biological samples with quantitative PCR as a reference method. Limit of detection (analytical sensitivity) of 5 (AdV, HEV, and RVA) and 50 (HAV and NoV) genome equivalents per reaction was reached. This robust, senstivie, and rapid multiplexing technology may be used to routinely monitor and manage viruses in food and water to prevent food and waterborne diseases.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
The Journal of Molecular Diagnostics
ISSN
1525-1578
e-ISSN
1943-7811
Volume of the periodical
23
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
765-776
UT code for WoS article
000654338000011
EID of the result in the Scopus database
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