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MOL-PCR and xMAP Technology: A Multiplex System for Fast Detection of Food- and Waterborne Viruses

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000078" target="_blank" >RIV/00027162:_____/21:N0000078 - isvavai.cz</a>

  • Alternative codes found

    RIV/60162694:G32__/21:N0000003 RIV/00216224:14310/21:00122387 RIV/62157124:16270/21:43879156

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S1525157821000866?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1525157821000866?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.jmoldx.2021.03.005" target="_blank" >10.1016/j.jmoldx.2021.03.005</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    MOL-PCR and xMAP Technology: A Multiplex System for Fast Detection of Food- and Waterborne Viruses

  • Original language description

    Viruses are common causes of food- and water-borne diseases worldwide. Conventional identification of these pathogenic agents is based on cultivation, antigen detection, electron microscopy or standardized real-time polymerase chain reaction systems. Recent technological advancements in sample processing and detection methods are currently more and more focused on fast and robust analysis. Following this approach in this study, we utilized a rapid multiplexing technology, which can detect a broad spectrum of pathogenic viruses connected to food or water contamination. We have designed a new semi-quantitative magnetic bead - based multiplex panel for simultaneous detection of several targets in one reaction. The panel includes adenoviruses 40/41 (AdV), rotavirus A (RVA), norovirus (NoV), hepatitis E virus (HEV), hepatitis A virus (HAV) and target for external control of the system. To evaluate the detection panel, interlaboratory ring tests were performed in four independent laboratories and a detection limit of 5 × 100 (AdV, HEV and RVA) and 5 × 101 (HAV, NoV) of genome equivalents in reaction was reached. The utilized rapid multiplexing technology represents an acceptably robust and sensitive method. As the results can be obtained in approximately 5 hours, its application could be useful for routine monitoring and control of viruses in food and water safety management and also in the case of viral outbreaks.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10607 - Virology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    The Journal of Molecular Diagnostics

  • ISSN

    1525-1578

  • e-ISSN

    1943-7811

  • Volume of the periodical

    23

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    12

  • Pages from-to

    765-776

  • UT code for WoS article

    000654338000011

  • EID of the result in the Scopus database

    2-s2.0-85106392271