MOL-PCR and xMAP Technology: A Multiplex System for Fast Detection of Food- and Waterborne Viruses
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000078" target="_blank" >RIV/00027162:_____/21:N0000078 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G32__/21:N0000003 RIV/00216224:14310/21:00122387 RIV/62157124:16270/21:43879156
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1525157821000866?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1525157821000866?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jmoldx.2021.03.005" target="_blank" >10.1016/j.jmoldx.2021.03.005</a>
Alternative languages
Result language
angličtina
Original language name
MOL-PCR and xMAP Technology: A Multiplex System for Fast Detection of Food- and Waterborne Viruses
Original language description
Viruses are common causes of food- and water-borne diseases worldwide. Conventional identification of these pathogenic agents is based on cultivation, antigen detection, electron microscopy or standardized real-time polymerase chain reaction systems. Recent technological advancements in sample processing and detection methods are currently more and more focused on fast and robust analysis. Following this approach in this study, we utilized a rapid multiplexing technology, which can detect a broad spectrum of pathogenic viruses connected to food or water contamination. We have designed a new semi-quantitative magnetic bead - based multiplex panel for simultaneous detection of several targets in one reaction. The panel includes adenoviruses 40/41 (AdV), rotavirus A (RVA), norovirus (NoV), hepatitis E virus (HEV), hepatitis A virus (HAV) and target for external control of the system. To evaluate the detection panel, interlaboratory ring tests were performed in four independent laboratories and a detection limit of 5 × 100 (AdV, HEV and RVA) and 5 × 101 (HAV, NoV) of genome equivalents in reaction was reached. The utilized rapid multiplexing technology represents an acceptably robust and sensitive method. As the results can be obtained in approximately 5 hours, its application could be useful for routine monitoring and control of viruses in food and water safety management and also in the case of viral outbreaks.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
The Journal of Molecular Diagnostics
ISSN
1525-1578
e-ISSN
1943-7811
Volume of the periodical
23
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
765-776
UT code for WoS article
000654338000011
EID of the result in the Scopus database
2-s2.0-85106392271