Characterization of Two Historic Smallpox Specimens from a Czech Museum.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG33__%2F17%3AN0000003" target="_blank" >RIV/60162694:G33__/17:N0000003 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/17:00478813 RIV/61388963:_____/17:00478813 RIV/67985904:_____/17:00478813 RIV/00023272:_____/17:10133602 and 3 more
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580457/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580457/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/v9080200" target="_blank" >10.3390/v9080200</a>

Result language
angličtina
Original language name
Characterization of Two Historic Smallpox Specimens from a Czech Museum.
Original language description
Although smallpox has been known for centuries, the oldest available variola virus strains were isolated in the early 1940s. At that time, large regions of the world were already smallpox-free. Therefore, genetic information of these strains can represent only the very last fraction of a long evolutionary process. Based on the genomes of 48 strains, two clades are differentiated: Clade 1 includes variants of variola major, and clade 2 includes West African and variola minor (Alastrim) strains. Recently, the genome of an almost 400-year-old Lithuanian mummy was determined, which fell basal to all currently sequenced strains of variola virus on phylogenetic trees. Here, we determined two complete variola virus genomes from human tissues kept in a museum in Prague dating back 60 and 160 years, respectively. Moreover, mass spectrometry-based proteomic, chemical, and microscopic examinations were performed. The 60-year-old specimen was most likely an importation from India, a country with endemic smallpox at that time. The genome of the 160-year-old specimen is related to clade 2 West African and variola minor strains. This sequence likely represents a new endemic European variant of variola virus circulating in the midst of the 19th century in Europe.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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