The heat-shock protein ClpB of Francisella tularensis is involved in stress tolerance and is required for multiplication in target organs of infected mice

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F08%3A00002012" target="_blank" >RIV/60162694:G44__/08:00002012 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The heat-shock protein ClpB of Francisella tularensis is involved in stress tolerance and is required for multiplication in target organs of infected mice

Original language description

Intracellular bacterial pathogens generally express chaperones such as Hsp100s during multiplication in host cells, allowing them to survive potentially hostile conditions. Francisella tularensis is a highly infectious bacterium causing the zoonotic disease tularaemia. The ability of F. tularensis to multiply and survive in macrophages is considered essential for its virulence. Although previous mutant screens in Francisella have identified the Hsp100 chaperone ClpB as important for intracellular survival, no detailed study has been performed. We demonstrate here that ClpB of F. tularensis live vaccine strain (LVS) is important for resistance to cellular stress. Promoter analysis shows that the transcriptional start is preceded by a sigma32-like promoter sequence and we demonstrate that expression of clpB is induced by heat shock. This indicates that expression of clpB is dependent on the heat-shock response mediated by sigma32, the only alternative sigma-factor present in Francisella.

Czech name

Protein tepelného šoku ClpB Franciselly tularensis se podílí na vzniku stresové tolerance a je nutný pro multiplikaci v cílových orgámnech infikovaných myší

Czech description

Předložená studie dokumentuje, že šaperon ClpB je významný pro rezistenci mikroba F. tularensis live vaccine strain (LVS) na buněčný stres. Analýza promotorové oblasti pak ukázala, že start transkripce předchází promotorová sekvence podobná sigma32 sekvenci. Tento nález indikuje, že produkce clpB je závislá na reakci na tepelný šok zprostředkované faktorem sigma32. Dále naše studie prokazuje, že ClpB přispívá k nitrobuněčné multiplikaci in vitro a je naprosto nezbytný pro replikaci mikroba v cílových orgánech a rovněž indukuje vznik onemocnění v myších.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/OPUOFVZ200602" target="_blank" >OPUOFVZ200602: Virulence factors of intracellular pathogen Francisella tularensis classified as cathegory A biological warfare agents</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Microbiology

ISSN

0950-382X

e-ISSN

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Volume of the periodical

67

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

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UT code for WoS article

000253633000016

EID of the result in the Scopus database

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