Voltammetric biosensor based on acetylcholinesterase and different immobilizaton protocols: a simple tool for toxic organophosphate assay

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002509" target="_blank" >RIV/60162694:G44__/11:00002509 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1080/00032719.2010.511745" target="_blank" >http://dx.doi.org/10.1080/00032719.2010.511745</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Voltammetric biosensor based on acetylcholinesterase and different immobilizaton protocols: a simple tool for toxic organophosphate assay
Original language description
The present study is aimed at construction of biosensors consisting of a screen printed sensor with a platinum working electrode and immobilized acetylcholinesterase (AChE) as a biorecognition element. Several immobilization protocols such as precipitation with glutaraldehyde, immobilization onto spherical graphite microparticles, sorption, interception into gelatin, and sol-gel were used in order to construct biosensors suitable for practical performance. The activity of AChE was evaluated by means ofvoltammetric oxidation of thiocholine originating from acetylthiocholine. Square wave and cyclic voltammetry were used throughout the experiments. Suitability of the constructed biosensors was evaluated using the toxic organophosphate diisopropylfluorophosphate.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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