Voltammetric Biosensor Based on Acetylcholinesterase and Different Immobilization Protocols: A Simple Tool for Toxic Organophosphate Assay
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F11%3A43870596" target="_blank" >RIV/62157124:16270/11:43870596 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/11:10118898
Result on the web
<a href="http://dx.doi.org/10.1080/00032719.2010.511745" target="_blank" >http://dx.doi.org/10.1080/00032719.2010.511745</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/00032719.2010.511745" target="_blank" >10.1080/00032719.2010.511745</a>
Alternative languages
Result language
angličtina
Original language name
Voltammetric Biosensor Based on Acetylcholinesterase and Different Immobilization Protocols: A Simple Tool for Toxic Organophosphate Assay
Original language description
The present study is aimed at construction of biosensors consisting of a screen printed sensor with a platinum working electrode and immobilized acetylcholinesterase (AChE) as a biorecognition element. Several immobilization protocols such as precipitation with glutaraldehyde, immobilization onto spherical graphite microparticles, sorption, interception into gelatin, and sol-gel were used in order to construct biosensors suitable for practical performance. The activity of AChE was evaluated by means ofvoltammetric oxidation of thiocholine originating from acetylthiocholine. Square wave and cyclic voltammetry were used throughout the experiments. Suitability of the constructed biosensors was evaluated using the toxic organophosphate diisopropylfluorophosphate.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Letters
ISSN
0003-2719
e-ISSN
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Volume of the periodical
44
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
1254-1264
UT code for WoS article
000291643600008
EID of the result in the Scopus database
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