Colorimetric glucose assay based on magnetic particles having pseudo-peroxidase activity and immobilized glucose oxidase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F16%3A43875583" target="_blank" >RIV/60162694:G44__/16:43875583 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16370/16:43874614

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs12033-016-9936-z" target="_blank" >http://link.springer.com/article/10.1007%2Fs12033-016-9936-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12033-016-9936-z" target="_blank" >10.1007/s12033-016-9936-z</a>

Result language

angličtina

Original language name

Colorimetric glucose assay based on magnetic particles having pseudo-peroxidase activity and immobilized glucose oxidase

Original language description

Magnetic particles (MPs) are currently used as a suitable alternative for peroxidase in the construction of novel biosensors, analytic and diagnostic methods. Their better chemical and thermal stabilities predestine them as appropriate pseudo-enzymatic catalysts. In this point of view, our research was focused on preparation of simply and fast method for immobilization of glucose oxidase onto surface of MPs with peroxidase-like activity. Spectrophotometric method (wavelength 450 nm) optimized for glucose determination using modified MPs has been successfully developed. Concentration curve for optimization of method was assayed, and Michaelis-Menten constant (K (m)) calculated, maximum reaction rate (V (max)), limit of detection, and correlation coefficient were determined to be 0.13 mmol/l (2.34 mg/dl), 1.79 pkat, 3.74 A mu mol/l (0.067 mg/dl), and 0.996, respectively. Interferences of other sugars such as sucrose, sorbitol, deoxyribose, maltose, and fructose were determined as well as effect of substances presenting in plasma (ascorbic acid, reduced glutathione, trolox, and urea). Results in comparison with positive and negative controls showed no interferences of the other sugars and no influence of plasma substances to measuring of glucose. The constructed method showed corresponding results with linear dependence and a correlation coefficient of 0.997. Possibility of repeated use of modified MPs was successfully proved.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Biotechnology

ISSN

1073-6085

e-ISSN

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Volume of the periodical

58

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

373-380

UT code for WoS article

000374286500007

EID of the result in the Scopus database

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