Determination of peroxidase-like activity of magnetic particles: basic platforms for peroxidase biosensors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F15%3A43875477" target="_blank" >RIV/60162694:G44__/15:43875477 - isvavai.cz</a>

Result on the web

<a href="http://www.electrochemsci.org/papers/vol10/100907033.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol10/100907033.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of peroxidase-like activity of magnetic particles: basic platforms for peroxidase biosensors

Original language description

Magnetic particles (MPs) are a current mean suitable for separation, biosensors construction, nanomedicine etc. They can be easily covered with a biomolecule and used for analytical purposes. Many enzymological assays have to be combined with use peroxidase. In this point of view, glucose assay by glucose oxidase can be exampled. In the present work, we focused our attention on the determination whether MPs based on iron oxide have pseudo-peroxidase activity allowing use them directly without linking to peroxidase. Hydrogen peroxide (H2O2) as the very common substrate of peroxidase was used for this measurement. Michaelis-Menten kinetics was performed for demonstration of catalytic activity of MPs by measuring various concentrations of H2O2 and square wave voltammetry (SWV), cyclic voltammetry (CV) and spectrophotometry were performed for the determination of pseudo-peroxidase activity. The used MPs had significant pseudo-peroxidase activity and Michaelis-Menten like behavior was observed and kinetics constants were determined. Michaelis-Menten constant (Km) was equal to 156.6 - 199.1 mmol/l (values are slightly different for the used analytical methods). Sensitivity of electrochemical and optical methods was also compared resulting in limits of detection found to be 0.42 mmol/l, 0.36 mmol /l and 13.1 mmol /l for SWV, CV and spectrophotometry respectively. In a conclusion, iron oxide MPs have high pseudo-peroxidase activity resulting in opportunity to use them for the determination of hydrogen peroxide. If an oxidase applied as a biorecognition element, there is no need to co-immobilize peroxidase. Km values were also determined for HRP using spectrophotometry (25.1 mmol/l), SWV (167. mmol/l) and CV (163.2 mmol/l).

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

July

Country of publishing house

CS - SERBIA AND MONTENEGRO

Number of pages

16

Pages from-to

7033-7048

UT code for WoS article

000365101700014

EID of the result in the Scopus database

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