Voltammetric biosensor based on a modified chitosan membrane enzyme peroxidase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F16%3A43875672" target="_blank" >RIV/60162694:G44__/16:43875672 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43410/16:43910861

Result on the web

<a href="http://www.electrochemsci.org/papers/vol11/111210391.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol11/111210391.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2016.12.64" target="_blank" >10.20964/2016.12.64</a>

Result language

angličtina

Original language name

Voltammetric biosensor based on a modified chitosan membrane enzyme peroxidase

Original language description

The determination of hydrogen peroxide (H2O2) has a significant meaning in environmental, pharmaceutical, healthcare and food analyses. The current work is focused on construction of a fast but reliable and accurate voltammetric biosensor enabling detection of H2O2 giving opportunity to its application into practice. This biosensor was based on screen printed electrode covered with chitosan membrane entrapping horseradish peroxidase (HRP), magnetic particles (MPs) and Prussian blue (PB). MPs an inorganic substrate having pseudo-peroxidase activity together with HRP catalyzed reduction of H2O2 and PB as mediator of electron transfer enabled highly sensitive detection at low applied potential. Square wave voltammetry (SWV) was chosen as a detection device. Average area peaks of each H2O2 concentration created calibration curve replying Michaelis-Menten equation with correlation coefficient 0.999. Limit of detection was equal to 8.2 µmol/l of H2O2. Uric acid, ascorbic acid, water soluble derivate of vitamin E (trolox), acetaminophen and reduced glutathione were measured as possibly interfering substances replacing H2O2 in the reaction and no significant influence on the assay was observed when presented in equivalent concentrations as the H2O2. Different personal care matrix substances (tooth paste, hand cream and skin tonic) were spiked with H2O2 and no effect of matrix on the determination was detected. On the basis of the presented results, , the proposed assay was considered to be highly sensitive, accurate and fast assay for detection of H2O2 so this platform can be applied in wide spectrum of research and clinical fields.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

12

Country of publishing house

CS - SERBIA AND MONTENEGRO

Number of pages

16

Pages from-to

10391-10406

UT code for WoS article

000391039000050

EID of the result in the Scopus database

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