Isolation of Amaryllidaceae alkaloids from Nerine bowdenii W. Watson and their biological activities

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F16%3A43875630" target="_blank" >RIV/60162694:G44__/16:43875630 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/16:70719 RIV/00216208:11160/16:10328751 RIV/00216275:25310/16:39901727

Result on the web

<a href="http://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C6RA20205E#!divAbstract" target="_blank" >http://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C6RA20205E#!divAbstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c6ra20205e" target="_blank" >10.1039/c6ra20205e</a>

Result language

angličtina

Original language name

Isolation of Amaryllidaceae alkaloids from Nerine bowdenii W. Watson and their biological activities

Original language description

Twenty-two isoquinoline alkaloids (1-22) were isolated from fresh bulbs of Nerine bowdenii (Amaryllidaceae) by standard chromatographic methods. The chemical structures were elucidated by MS, and 1D and 2D NMR spectroscopic analyses, and by comparison with literature data. 6-O-Demethylbelladine (11) and 4'-O-demethylbelladine (12) are reported here for the first time. Compounds isolated in sufficient amounts were evaluated for their acetylcholinesterase, and butyrylcholinesterase inhibition activity using Ellman's method. In the prolyl oligopeptidase assay, Z-Gly-Pro-p-nitroanilide was used as substrate. Untested alkaloids were also screened for their cytotoxic activity against p53-mutated Caco-2 and HT-29 colorectal adenocarcinoma cells. At the same time, healthy small intestine cells FH-74 Int were used to determine overall toxicity against noncancerous cells. The crinine-type alkaloid buphanisine (7) demonstrated interesting cytotoxicity against both tested cancer cell lines with IC50 values of 8.59 +/- 0.15 mu M for Caco-2 and 5.32 +/- 1.70 mu M for HT-29.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

RSC Advances

ISSN

2046-2069

e-ISSN

—

Volume of the periodical

6

Issue of the periodical within the volume

83

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

80114-80120

UT code for WoS article

000382539600098

EID of the result in the Scopus database

—