Colorimetric sol gel based biosensor platform for determination of reduced glutathione

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F16%3A43875670" target="_blank" >RIV/60162694:G44__/16:43875670 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43410/16:43910864

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0925400516308644" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0925400516308644</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2016.06.012" target="_blank" >10.1016/j.snb.2016.06.012</a>

Result language

angličtina

Original language name

Colorimetric sol gel based biosensor platform for determination of reduced glutathione

Original language description

Reduced glutathione (GSH) is a biochemical and antioxidant marker in various examinations of pathological states. Though standard tests for determination GSH in biological samples are available, there are significant drawbacks. The method for determination of GSH based on enzymatic reaction applying enzyme glutathione-S-transferase (GST) was established and introduced to provide a simple but reliable platform for determination of GSH in biological samples. Sol-gel membrane was utilized for immobilization of the homogenate from pig liver containing high activity of GST. Enzymatic method was compared with currently used Ellman method. The results of experiments showed, that Ellman method as well as enzymatic sol-gel method are appropriate for the determination of GSH in tissues. Michaelis-Menten constant of the novel method was set to be 1.9 mmol/l and limit of detection (LOD) of Ellman method was determined to be 30 mu mol/l while LOD of enzymatic method was set to be six times higher. Concerning to interferences, enzymatic sol-gel technique showed better results compared to the standard Ellman method. No interfering substances were revealed in the GST based assay. On the other hand, bovine serum albumin, ascorbic acid (AA) and N-acetylcysteine (N-AC) were confirmed as strong interferents in Ellman method. Spiked plasma samples were measured using the GST based method and their kinetic curve has very similar shape as kinetic curve of standard solutions.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors and Actuators B-Chemical

ISSN

0925-4005

e-ISSN

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Volume of the periodical

236

Issue of the periodical within the volume

June

Country of publishing house

CH - SWITZERLAND

Number of pages

8

Pages from-to

442-449

UT code for WoS article

000382229700053

EID of the result in the Scopus database

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