Piezoelectric biosensor for the determination of Tumor Necrosis Factor Alpha
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F18%3A43889330" target="_blank" >RIV/60162694:G44__/18:43889330 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0039914017310731" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0039914017310731</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2017.10.031" target="_blank" >10.1016/j.talanta.2017.10.031</a>
Alternative languages
Result language
angličtina
Original language name
Piezoelectric biosensor for the determination of Tumor Necrosis Factor Alpha
Original language description
Tumor Necrosis Factor Alpha (TNF alpha) is an important marker of inflammatory processes in human body. In the current healthcare, determination of TNFa blood or plasma level is done by Enzyme Linked Immuno-Sorbent Assay (ELISA) as a primary choice method. Piezoelectric immunosensors are analytical platform recording affinity interactions on their surface. It is inferred that the immunosensors would be a functional alternative to the ELISA. In this study, antibody against TNF alpha was immobilized on Quartz Crystal Microbalance (QCM) sensor and the same was made on magnetic particles. Human TNFa was measured in a way of interaction with QCM surface and then the particles were applied. The assay exerted sufficient limit of detection equal to 1.62 pg/ml and it also fully correlated with standard ELISA tests. No interference by interleukin 6 or albumin was observed. Long term stability of the immunosensors lasting for at least three months was found. The immunosensors appears to be readily for practical performance and it would be an alternative to the standard ELISA especially when diagnoses made in field, homecare conditions or conditions of small hospitals as an emergency test.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Talanta
ISSN
0039-9140
e-ISSN
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Volume of the periodical
178
Issue of the periodical within the volume
February
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
4
Pages from-to
970-973
UT code for WoS article
000416615500129
EID of the result in the Scopus database
2-s2.0-85032383725