Immunoassay of interferon gamma by quartz crystal microbalance biosensor
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00556106" target="_blank" >RIV/60162694:G44__/20:00556106 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0039914020304586?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0039914020304586?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2020.121167" target="_blank" >10.1016/j.talanta.2020.121167</a>
Alternative languages
Result language
angličtina
Original language name
Immunoassay of interferon gamma by quartz crystal microbalance biosensor
Original language description
Interferon gamma (IFN gamma) is a cytokine and an immunochemical marker that can be used for revealing of infectious diseases and especially for distinguishing of viral and some types of bacterial infections. Blood tests for IFN gamma are typically based on immunoassays like Enzyme-Linked Immunosorbent Assay (ELISA). In this paper, a biosensor working on the principle of quartz crystal microbalance (QCM) was developed as an alternative to the standard analytical methods for IFN gamma. The biosensor contained antibodies against IFN gamma immobilized on QCM and also on gold nanoparticles. A sandwich containing QCM, gold nanoparticles and IFN gamma was formed and formation of the sandwich caused decrease of oscillation frequency. The assay exerted limit of detection 5.7 pg/ml for a sample sized 50 mu l and one measuring cycle was finished within 90 min. The assay by biosensor fully correlated to standard ELISA. In a conclusion, the biosensor appears to be a fully applicable analytical tool for a simple assay of IFN gamma. Overall simplicity and no special requirement on staff and equipment are the major advantages of the here presented assay.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Talanta
ISSN
0039-9140
e-ISSN
1873-3573
Volume of the periodical
218
Issue of the periodical within the volume
Oct
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
5
Pages from-to
121167
UT code for WoS article
000564486000009
EID of the result in the Scopus database
2-s2.0-85085258542