Construction of a QCM Biosensor for free Hemoglobin Assay

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00536959" target="_blank" >RIV/60162694:G44__/19:00536959 - isvavai.cz</a>
Result on the web
<a href="http://www.electrochemsci.org/papers/vol14/140605237.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol14/140605237.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.20964/2019.06.48" target="_blank" >10.20964/2019.06.48</a>

Result language
angličtina
Original language name
Construction of a QCM Biosensor for free Hemoglobin Assay
Original language description
Protein responsible for oxygen transfer, hemoglobin, is an important marker in biochemistry that can be measured in blood and blood plasma. Meaning of the assays are different in the two fluids. While blood hemoglobin serving as a marker of anemia and some oncotic processes is simply measurable by spectroscopy, hemoglobin content in plasma, a marker of hemolytic processes, is significantly lower and the assay should be done by more elaborative techniques. In this paper, piezoelectric biosensor containing QCM platform with basic frequency of oscillations 10 MHz and gold circle electrodes on the opposite sites, antibody against hemoglobin and iron oxide nanoparticles as blocker and antibody layer stabilizer was prepared. The assay exerted limit of detection equal to 0.063 mg/ml and the calibration well correlated (r(2)= 0.995) with standard ELISA. No interference by immunoglobulins and albumin was proved. The assay appears to applicable for clinical praxis and outside of central hospital laboratories. Reasonably limit of detection, low price of the biosensor and device, simple procedure of sample use, no necessity of specific reagents and label free type of assay are the major advantages of the biosensor.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)