Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for identification of strictly anaerobic bacterium Zymophilus.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F15%3A%230001109" target="_blank" >RIV/60193697:_____/15:#0001109 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/15:00463851
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S1075996415000256" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1075996415000256</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.anaerobe.2015.02.004" target="_blank" >10.1016/j.anaerobe.2015.02.004</a>
Alternative languages
Result language
angličtina
Original language name
Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for identification of strictly anaerobic bacterium Zymophilus.
Original language description
PCR-primers were designed for identification of strictly anaerobic bacteria of the genus Zymophilus based on genus-specific sequences of the 16S-23S rDNA internal transcribed spacer region. The specificity of the primers was tested against 37 brewery-related non-target microorganisms that could potentially occur in the same brewery specimens. None DNA was amplified from any of the non-Zymophilus strains tested including genera from the same family (Pectinatus, Megasphaera, Selenomonas), showing thus 100% specificity. PCR assay developed in this study allows an extension of the spectra of detected beer spoilage microorganisms in brewery laboratories.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GAP503%2F12%2F1424" target="_blank" >GAP503/12/1424: Anaerobic food spoiling bacteria and their biofilm forming potential</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Anaerobe
ISSN
1075-9964
e-ISSN
—
Volume of the periodical
33
Issue of the periodical within the volume
June 2015
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
5
Pages from-to
85-89
UT code for WoS article
—
EID of the result in the Scopus database
—