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Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for identification of strictly anaerobic bacterium Zymophilus.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F15%3A%230001109" target="_blank" >RIV/60193697:_____/15:#0001109 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/15:00463851

  • Result on the web

    <a href="http://www.sciencedirect.com/science/article/pii/S1075996415000256" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1075996415000256</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.anaerobe.2015.02.004" target="_blank" >10.1016/j.anaerobe.2015.02.004</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for identification of strictly anaerobic bacterium Zymophilus.

  • Original language description

    PCR-primers were designed for identification of strictly anaerobic bacteria of the genus Zymophilus based on genus-specific sequences of the 16S-23S rDNA internal transcribed spacer region. The specificity of the primers was tested against 37 brewery-related non-target microorganisms that could potentially occur in the same brewery specimens. None DNA was amplified from any of the non-Zymophilus strains tested including genera from the same family (Pectinatus, Megasphaera, Selenomonas), showing thus 100% specificity. PCR assay developed in this study allows an extension of the spectra of detected beer spoilage microorganisms in brewery laboratories.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GAP503%2F12%2F1424" target="_blank" >GAP503/12/1424: Anaerobic food spoiling bacteria and their biofilm forming potential</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Anaerobe

  • ISSN

    1075-9964

  • e-ISSN

  • Volume of the periodical

    33

  • Issue of the periodical within the volume

    June 2015

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    5

  • Pages from-to

    85-89

  • UT code for WoS article

  • EID of the result in the Scopus database