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Development of a species-specific PCR assay for identification of the strictly anaerobic bacterium Selenomonas lacticifex found in biofilm-covered surfaces in brewery bottling halls

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00506696" target="_blank" >RIV/61388971:_____/14:00506696 - isvavai.cz</a>

  • Alternative codes found

    RIV/60193697:_____/14:#0000967

  • Result on the web

    <a href="https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.12610" target="_blank" >https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.12610</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/jam.12610" target="_blank" >10.1111/jam.12610</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Development of a species-specific PCR assay for identification of the strictly anaerobic bacterium Selenomonas lacticifex found in biofilm-covered surfaces in brewery bottling halls

  • Original language description

    AimsIn recent years, beer-spoilage cases from strictly anaerobic bacteria have risen in frequency, in connection with the production of non-pasteurized, non-alcohol and low-alcoholic beers and with the lowering of dissolved oxygen in the packaged beer. Selenomonas lacticifex, found in brewer's yeast and in biofilms covering some surfaces in brewery bottling area, is considered to be a beer-spoilage organism. This study aims to develop S.lacticifex-specific PCR assay. The objective of this study was also evaluation of the specificity and reproducibility of the developed PCR assay in real brewery samples. nMethods and ResultsThree primers (one forward and two reverse) were designed for identification of the strictly anaerobic bacterium S.lacticifex on the basis of the species-specific sequences of the 16S rDNA region. The specificity of the primers was tested against 44 brewery-related non-target micro-organisms that could potentially occur in the same brewery specimens. None of the primer pairs amplified DNA from any of the non-S.lacticifex strains tested including genera from the same family (Pectinatus, Megasphaera, Zymophilus) and the closely related species Selenomonas ruminantium, showing thus 100% specificity. nConclusionsThe PCR assay developed in this study enables the detection of the strictly anaerobic bacterium S.lacticifex in real brewery samples including pitching yeast. nSignificance and Impact of the StudySelenomonas lacticifex-specific PCR assay developed in this study allows for the extension of the spectra of detected beer-spoilage micro-organisms in brewing laboratories and thus lowering the risk of contamination of the final product.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Applied Microbiology

  • ISSN

    1364-5072

  • e-ISSN

  • Volume of the periodical

    117

  • Issue of the periodical within the volume

    5

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    1328-1335

  • UT code for WoS article

    000343870700011

  • EID of the result in the Scopus database

    2-s2.0-84908013133