Development of a species-specific PCR assay for identification of the strictly anaerobic bacterium Selenomonas lacticifex found in biofilm-covered surfaces in brewery bottling halls
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00506696" target="_blank" >RIV/61388971:_____/14:00506696 - isvavai.cz</a>
Alternative codes found
RIV/60193697:_____/14:#0000967
Result on the web
<a href="https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.12610" target="_blank" >https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.12610</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jam.12610" target="_blank" >10.1111/jam.12610</a>
Alternative languages
Result language
angličtina
Original language name
Development of a species-specific PCR assay for identification of the strictly anaerobic bacterium Selenomonas lacticifex found in biofilm-covered surfaces in brewery bottling halls
Original language description
AimsIn recent years, beer-spoilage cases from strictly anaerobic bacteria have risen in frequency, in connection with the production of non-pasteurized, non-alcohol and low-alcoholic beers and with the lowering of dissolved oxygen in the packaged beer. Selenomonas lacticifex, found in brewer's yeast and in biofilms covering some surfaces in brewery bottling area, is considered to be a beer-spoilage organism. This study aims to develop S.lacticifex-specific PCR assay. The objective of this study was also evaluation of the specificity and reproducibility of the developed PCR assay in real brewery samples. nMethods and ResultsThree primers (one forward and two reverse) were designed for identification of the strictly anaerobic bacterium S.lacticifex on the basis of the species-specific sequences of the 16S rDNA region. The specificity of the primers was tested against 44 brewery-related non-target micro-organisms that could potentially occur in the same brewery specimens. None of the primer pairs amplified DNA from any of the non-S.lacticifex strains tested including genera from the same family (Pectinatus, Megasphaera, Zymophilus) and the closely related species Selenomonas ruminantium, showing thus 100% specificity. nConclusionsThe PCR assay developed in this study enables the detection of the strictly anaerobic bacterium S.lacticifex in real brewery samples including pitching yeast. nSignificance and Impact of the StudySelenomonas lacticifex-specific PCR assay developed in this study allows for the extension of the spectra of detected beer-spoilage micro-organisms in brewing laboratories and thus lowering the risk of contamination of the final product.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Applied Microbiology
ISSN
1364-5072
e-ISSN
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Volume of the periodical
117
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
1328-1335
UT code for WoS article
000343870700011
EID of the result in the Scopus database
2-s2.0-84908013133