Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for the detection and identification of strictly anaerobic bacterium Zymophilus (poster)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F14%3A%230001019" target="_blank" >RIV/60193697:_____/14:#0001019 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for the detection and identification of strictly anaerobic bacterium Zymophilus (poster)
Original language description
PCR-primers were designed for identification of the strictly anaerobic bacterium Zymophilus on the basis of the genus-specific sequences of the 16S-23S rDNA internal transcribed spacer region. The specificity of the primers was tested against 37 brewery-related non-target microorganisms that could potentially occur in the same brewery specimens. None DNA was amplified from any of the non-Zymophilus strains tested including genera from the same family (Pectinatus, Megasphaera, Selenomonas), showing thus100% specificity. PCR assay developed in this study allows for the extension of the spectra of detected beer spoilage microorganisms in brewery laboratories. 37th Brewing and Malting Conference. Pilsen, Czech Republic, October 23-24, 2014.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP503%2F12%2F1424" target="_blank" >GAP503/12/1424: Anaerobic food spoiling bacteria and their biofilm forming potential</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů