Ultrastruktural Localization of Calcium Deposits in Pig Oocytes Maturing in Vitro: Effects of Verapamil

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F03%3A00005585" target="_blank" >RIV/60460709:41210/03:00005585 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Ultrastruktural Localization of Calcium Deposits in Pig Oocytes Maturing in Vitro: Effects of Verapamil
Original language description
The culture of pig oocytes in the presence of the calcium channel blocker verapamil (0,02 mM) resulted in the blocking of meiosis at the metaphase I stage, and only a small fraction (about 28%) of the oocytes were able to continue their maturation to thestage of metaphase II. Hence, meiotic maturation in pig oocytes is a calcium-dependent process. The amount of calcium deposits in the oocyte and granulose cells increased during oocyte meiotic maturation in vitro, especially in the nucleus, mitochondria, vacules, and cytoplasm. The replenishment of calcium deposits is significantly changed under the effect of verapamil. The increase of calcium deposits in the oocyte nucleus was dalayed. A much larger amount of deposits was demonstratively smaller. A significant peak in the accumulation of calcium deposits was observed in the cytoplasm of verapamil-treated oocytes after 16 hours of in vitro culture. We propose that an altered pattern in the replenishment of calcium deposits can disturb
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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