Enumeration, isolation and identification of bifidobacteria from infant feces.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A7069" target="_blank" >RIV/60460709:41210/04:7069 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Enumeration, isolation and identification of bifidobacteria from infant feces.

Original language description

The aim of our work was to enumerate, isolate and identify bifidobacteria in infant faeces. Thirty three fully breast-fed infants in the age between 1 and 12 weeks were investigated. FISH kit for the quantitative detection of Bifidobacterium sp. was compared with culture method. Bifidobacteria in faecal samples were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg/L) and glacial acetic acid (1 ml/L). Pure cultures were identified to the genus level using fructose-6-phosphate phosphoketolase test, and to the species level using API 50 CHL and API ID 32 A Rapid tests followed by computer program Bacter. The identification to the species level was also carried out by PCR method using species-specific primers. Bifidobacteria counts in most faecal samples determined by both TPY agar and FISH procedure were from 108 to 1011 CFU/g. Three infants did not contain any bifidobacteria in their faecal samples. One of them was delivered by caesarean section and tw

Czech name

Stanovení počtu a isolace a identifikace bifidobakterií z kojeneckých výkalů.

Czech description

The aim of our work was to enumerate, isolate and identify bifidobacteria in infant faeces. Thirty three fully breast-fed infants in the age between 1 and 12 weeks were investigated. FISH kit for the quantitative detection of Bifidobacterium sp. was compared with culture method. Bifidobacteria in faecal samples were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg/L) and glacial acetic acid (1 ml/L). Pure cultures were identified to the genus level using fructose-6-phosphate phosphoketolase test, and to the species level using API 50 CHL and API ID 32 A Rapid tests followed by computer program Bacter. The identification to the species level was also carried out by PCR method using species-specific primers. Bifidobacteria counts in most faecal samples determined by both TPY agar and FISH procedure were from 108 to 1011 CFU/g. Three infants did not contain any bifidobacteria in their faecal samples. One of them was delivered by caesarean section and tw

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

2

Issue of the periodical within the volume

49

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

209-212

UT code for WoS article

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EID of the result in the Scopus database

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