Enumeration, isolation, and identification of bifidobacteria from dairy products.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A7932" target="_blank" >RIV/60460709:41210/04:7932 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Enumeration, isolation, and identification of bifidobacteria from dairy products.

Original language description

Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg/l). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) andby the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46°C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification wasalso carried out by PCR method using genus- and species-specific primers. The bifidobacterial counts in products tested varied from 2.37 to 7.17 log CFU/ml. High selectivity was seen for modified TPY agar from which all isolates were identified as bifidobacteria. While most strains were identified as B. animalis using Bacter program, the same isolates had positive reaction with B. lactis-specific primers. Strain isolated from Rajo yogurt was identified as B. longum by Bacter as well as

Czech name

Počty, isolace a identifikace bifidobakterií izolovaných z mléčných výrobků.

Czech description

Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg/l). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) andby the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46°C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification wasalso carried out by PCR method using genus- and species-specific primers. The bifidobacterial counts in products tested varied from 2.37 to 7.17 log CFU/ml. High selectivity was seen for modified TPY agar from which all isolates were identified as bifidobacteria. While most strains were identified as B. animalis using Bacter program, the same isolates had positive reaction with B. lactis-specific primers. Strain isolated from Rajo yogurt was identified as B. longum by Bacter as well as

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Agriculturae Slovenica

ISSN

1581-9175

e-ISSN

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Volume of the periodical

1

Issue of the periodical within the volume

84

Country of publishing house

SI - SLOVENIA

Number of pages

6

Pages from-to

31-36

UT code for WoS article

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EID of the result in the Scopus database

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