Detection of faecal bacteria by FISH method.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F06%3A17258" target="_blank" >RIV/60460709:41210/06:17258 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detection of faecal bacteria by FISH method.

Original language description

Cultivation methods for the quantitative detection of the faecal bacteria are inaccurate and time consuming, also not all bacteria are cultivatable and results obtained may be incorrect. Hence, the aim of our study was to evaluate FISH method for the quantitative detection of different bacterial group in faecal samples of infants, calves, and lambs during the milk-feeding period. Faecal samples from 91 infants, 20 calves, and 18 lambs were tested. A specific fluorescence in situ hybridisation (FISH) kitfor Bifidobacterium spp., Clostridium butyricum group, Escherichia coli, and Lactobacillus spp. were used for the quantitative detection of bacteria compared to cultivation methods. Sixty-nine infants possessed high numbers of bifidobacteria in their faeces. Cultivation counts were nonsignificantly lower (9.91 ? 0.67) than counts determinated by FISH (9.92 ? 0.55). Twenty-two infants did not contain detectable bifidobacteria. These infants possessed as a major group of their faecal f

Czech name

Detekce fekálních bakterií FISH metodou.

Czech description

Cultivation methods for the quantitative detection of the faecal bacteria are inaccurate and time consuming, also not all bacteria are cultivatable and results obtained may be incorrect. Hence, the aim of our study was to evaluate FISH method for the quantitative detection of different bacterial group in faecal samples of infants, calves, and lambs during the milk-feeding period. Faecal samples from 91 infants, 20 calves, and 18 lambs were tested. A specific fluorescence in situ hybridisation (FISH) kitfor Bifidobacterium spp., Clostridium butyricum group, Escherichia coli, and Lactobacillus spp. were used for the quantitative detection of bacteria compared to cultivation methods. Sixty-nine infants possessed high numbers of bifidobacteria in their faeces. Cultivation counts were nonsignificantly lower (9.91 ? 0.67) than counts determinated by FISH (9.92 ? 0.55). Twenty-two infants did not contain detectable bifidobacteria. These infants possessed as a major group of their faecal f

Type

D - Article in proceedings

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GP523%2F05%2FP117" target="_blank" >GP523/05/P117: The occurrence of bifidobacteria and their role in young ruminants during the milk-feeding period</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Book of Abstracts Genetics of industrial microorganisms.

ISBN

80-239-7323-1

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

0-0

Publisher name

Institute of Microbiologzy ASCR

Place of publication

Prague

Event location

Prague

Event date

Jun 24, 2006

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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