Multiplex Analysis of Cytokines Involved in Tumour Growth and Spontaneous Regression in a Rat Sarcoma Model

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F07%3A23268" target="_blank" >RIV/60460709:41210/07:23268 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/07:00089246 RIV/67985904:_____/07:00089246

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Multiplex Analysis of Cytokines Involved in Tumour Growth and Spontaneous Regression in a Rat Sarcoma Model

Original language description

The aim of our study was to examine in vivo and in vitro cytokines produced by Lewis rat-derived R5-28 sarcoma cells. These cells produce rapidly growing tumours in approximately two weeks after subcutaneous inoculation. However, spontaneous tumour regression was noted in about 40% of animals. For an explanation of this phenomenon, we evaluated the profile of 19 cytokines during tumour growth and spontaneous regression by the use of antibody array. To detect cytokines directly originated by the sarcoma,the R5-28 cells were cultivated in vitro and then both the supernatants and the cell lysates were analysed. Our experiments showed three cytokines (MCP-1, TIMP-1 and VEGF) to be produced by R5-28 cells in vitro. Moreover, in vivo, other three cytokines(TNF-á, â-NGF and LIX) were detected both in blood sera and tumour lysates, probably produced by immune and stromal cells during tumour growth. Changes in their expression after spontaneous regression are discussed.

Czech name

Analýza cytokinů, které jsou zapojeny do růstu a samovolné regrese sarkomu u potkana

Czech description

The aim of our study was to examine in vivo and in vitro cytokines produced by Lewis rat-derived R5-28 sarcoma cells. These cells produce rapidly growing tumours in approximately two weeks after subcutaneous inoculation. However, spontaneous tumour regression was noted in about 40% of animals. For an explanation of this phenomenon, we evaluated the profile of 19 cytokines during tumour growth and spontaneous regression by the use of antibody array. To detect cytokines directly originated by the sarcoma,the R5-28 cells were cultivated in vitro and then both the supernatants and the cell lysates were analysed. Our experiments showed three cytokines (MCP-1, TIMP-1 and VEGF) to be produced by R5-28 cells in vitro. Moreover, in vivo, other three cytokines(TNF-á, â-NGF and LIX) were detected both in blood sera and tumour lysates, probably produced by immune and stromal cells during tumour growth. Changes in their expression after spontaneous regression are discussed.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Biologica - Prague

ISSN

0015-5500

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

53

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

216-219

UT code for WoS article

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EID of the result in the Scopus database

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