In vitro aging of pig oocytes: effects of the histone deacetylase inhibitor trichostatin A

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F08%3A26239" target="_blank" >RIV/60460709:41210/08:26239 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

In vitro aging of pig oocytes: effects of the histone deacetylase inhibitor trichostatin A

Original language description

After in vitro maturation, the unfertilized pig oocytes underwent the process called aging. This process involves typical events such as fragmentation, spontaneous parthenogenetic activation or lysis. Inhibition of histone deacetylase, using its specificinhibitor trichostatin A (TSA), significantly delayed the maturation of pig oocytes cultured in vitro. The aging of oocytes matured under the effect of TSA is the same as the aging in oocytes matured without TSA. The inhibition of histone deacetylase during oocyte aging significantly reduced the percentage of fragmented oocytes (from 30% in untreated oocytes to 9% in oocytes aged under the effect of 100 nM of TSA). Oocytes matured in vitro and subsequently aged for 1 day under the effects of TSA retained their developmental capacity. After parthenogenetic activation, a significantly higher portion (27% vs. 15%) of oocytes developed to the blastocyst stage after 24hour aging under100 nM TSA when compared to oocytes activated after 24hou

Czech name

n vitro stárnutí prasečích oocytů efekt inhibitoru histon deacetylázy trichostatinu A

Czech description

After in vitro maturation, the unfertilized pig oocytes underwent the process called aging. This process involves typical events such as fragmentation, spontaneous parthenogenetic activation or lysis. Inhibition of histone deacetylase, using its specificinhibitor trichostatin A (TSA), significantly delayed the maturation of pig oocytes cultured in vitro. The aging of oocytes matured under the effect of TSA is the same as the aging in oocytes matured without TSA. The inhibition of histone deacetylase during oocyte aging significantly reduced the percentage of fragmented oocytes (from 30% in untreated oocytes to 9% in oocytes aged under the effect of 100 nM of TSA). Oocytes matured in vitro and subsequently aged for 1 day under the effects of TSA retained their developmental capacity. After parthenogenetic activation, a significantly higher portion (27% vs. 15%) of oocytes developed to the blastocyst stage after 24hour aging under100 nM TSA when compared to oocytes activated after 24hou

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GG - Zootechnics

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Zygote

ISSN

0967-1994

e-ISSN

—

Volume of the periodical

16

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

18

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—