Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte aging in vitro
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F13%3A61524" target="_blank" >RIV/60460709:41210/13:61524 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte aging in vitro
Original language description
Oocyte aging is the complex of processes that occur when matured in vitro oocytes are exposed, after reaching the metaphase II stage further in the vitro culture. Aged oocytes remaining at the metaphase II stage, underwent spontaneous parthenogenetic activation, or cellular death, through apoptosis (fragmentation) or lysis. The key factor in apoptotic pathway regulation is c-Jun-terminal kinase (JNK), stress kinase from the MAPK kinase family. To investigate the effect of JNK inhibition on porcine oocytes aging, cleavage rate and embryonic development after parthenogenetic activation, DNA fragmentation, and pro-apoptotic factor Bax expression, we cultured in vitro matured oocytes for another 1 to 4 days in the presence of a JNK inhibitor. The inhibition of JNK significantly protected oocytes from the fragmentation (0.0 % of fragmented oocytes under JNK inhibition vs. 13.4% of fragmented oocytes in the control group, second day of aging) and increased the percentage of parthenogenetical
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GI - Farm animal breeding and farm animal pedigree breeding
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
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Volume of the periodical
58
Issue of the periodical within the volume
12
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
11
Pages from-to
535-545
UT code for WoS article
000328563000001
EID of the result in the Scopus database
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