Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F13%3A%230001922" target="_blank" >RIV/00027014:_____/13:#0001922 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/13:00398779

Result on the web

<a href="http://www.agriculturejournals.cz/publicFiles/106669.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/106669.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro

Original language description

Oocyte ageing is a complex of processes that occur when matured in vitro oocytes are, after reaching the metaphase II stage, exposed to further in vitro culture. Aged oocytes remaining at the metaphase II stage undergo spontaneous parthenogenetic activation, or cellular death, through apoptosis (fragmentation) or lysis. The key factor in apoptotic pathway regulation is c-Jun-N-terminal kinase (JNK), stress kinase from the mitogene-activated protein kinase (MAPK) family. To investigate the effect of JNKinhibition on porcine oocytes ageing, cleavage rate, and embryonic development after parthenogenetic activation, DNA fragmentation, and pro-apoptotic factor Bax expression, we cultured in vitro matured oocytes for another 1?4 days in the presence of a JNK inhibitor. The inhibition of JNK significantly protected the oocytes from fragmentation (0% of fragmented oocytes under JNK inhibition vs. 13,4% of fragmented oocytes in the control group, 2nd day of ageing) and increased the percentage

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

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Volume of the periodical

58

Issue of the periodical within the volume

12

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

535-545

UT code for WoS article

000328563000001

EID of the result in the Scopus database

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