Anti-apoptotic properties of carbon monoxide in porcine oocyte during in vitro aging

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F17%3A74346" target="_blank" >RIV/60460709:41210/17:74346 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.7717/peerj.3876" target="_blank" >http://dx.doi.org/10.7717/peerj.3876</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.7717/peerj.3876" target="_blank" >10.7717/peerj.3876</a>

Result language

angličtina

Original language name

Anti-apoptotic properties of carbon monoxide in porcine oocyte during in vitro aging

Original language description

If fertilization of matured oocyte does not occur, unfertilized oocyte undergoes aging, resulting in a time dependent reduction of the oocyte s quality. The aging of porcine oocytes can lead to apoptosis. Carbon monoxide (CO), a signal molecule produced by the heme oxygenase (HO), possesses cytoprotective and anti-apoptotic effects that have been described in somatic cells. However, the effects of CO in oocytes have yet to be investigated. By immunocytochemistry method we detected that both isoforms of heme oxygenase are present in the porcine oocytes. Based on the morphological signs of oocyte aging, it was found that the inhibition of both HO isoforms by Zn protoporphyrin IX leads to an increase in the number of apoptotic oocytes and decrease in the number of intact oocytes during aging. Contrarily, the presence of CO donors significantly decrease the number of apoptotic oocytes while increasing the number of intact oocytes. We also determined that CO donors significantly decrease the caspase3 (CAS

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

<a href="/en/project/QJ1510138" target="_blank" >QJ1510138: Innovation of reproductive biotechiques in farm animals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PeerJ

ISSN

2167-8359

e-ISSN

—

Volume of the periodical

5

Issue of the periodical within the volume

N

Country of publishing house

GB - UNITED KINGDOM

Number of pages

25

Pages from-to

1-25

UT code for WoS article

000412520500006

EID of the result in the Scopus database

2-s2.0-85030710158