The effect of carbon monoxide on meiotic maturation of porcine oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F21%3AN0000052" target="_blank" >RIV/00027014:_____/21:N0000052 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/21:85310

Result on the web

<a href="http://nih.gov" target="_blank" >http://nih.gov</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.7717/peerj.10636" target="_blank" >10.7717/peerj.10636</a>

Result language

angličtina

Original language name

The effect of carbon monoxide on meiotic maturation of porcine oocytes

Original language description

Oxidative stress impairs the correct course of meiotic maturation, and it is known that the oocytes are exposed to increased oxidative stress during meiotic maturation in in vitro conditions. Thus, reduction of oxidative stress can lead to improved quality of cultured oocytes. The gasotransmitter carbon monoxide (CO) has a cytoprotective effect in somatic cells. The CO is produced in cells by the enzyme heme oxygenase (HO) and the heme oxygenase/carbon monoxide (HO/CO) pathway has been shown to have an antioxidant effect in somatic cells. It has not yet been investigated whether the CO has an antioxidant effect in oocytes as well. We assessed the level of expression of HO mRNA, using reverse transcription polymerase chain reaction. The HO protein localization was evaluated by the immunocytochemical method. The influence of CO or HO inhibition on meiotic maturation was evaluated in oocytes cultured in a culture medium containing CO donor (CORM-2 or CORM-A1) or HO inhibitor Zn-protoporphyrin IX (Zn-PP IX). Detection of reactive oxygen species (ROS) was performed using the oxidant-sensing probe 2′,7′-dichlorodihydrofluorescein diacetate. We demonstrated the expression of mRNA and proteins of both HO isoforms in porcine oocytes during meiotic maturation. The inhibition of HO enzymes by Zn-PP IX did not affect meiotic maturation. CO delivered by CORM-2 or CORM-A1 donors led to a reduction in the level of ROS in the oocytes during meiotic maturation. However, exogenously delivered CO also inhibited meiotic maturation, especially at higher concentrations. In summary, the CO signaling molecule has antioxidant properties in porcine oocytes and may also be involved in the regulation of meiotic maturation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

<a href="/en/project/LTC18059" target="_blank" >LTC18059: Cultivation system suitable for long-term cultivation of human follicular cells</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PeerJ

ISSN

2167-8359

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

march

Country of publishing house

GB - UNITED KINGDOM

Number of pages

21

Pages from-to

Article number e10636

UT code for WoS article

000631683800001

EID of the result in the Scopus database

2-s2.0-85103122232