Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A77131" target="_blank" >RIV/60460709:41210/18:77131 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.17221/117/2018-CJAS" target="_blank" >http://dx.doi.org/10.17221/117/2018-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/117/2018-CJAS" target="_blank" >10.17221/117/2018-CJAS</a>
Alternative languages
Result language
angličtina
Original language name
Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes
Original language description
The aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact membranes (INT) in Holstein (4 bulls, 72 samples) and Czech Fleckvieh (4 bulls, 72 samples) semen frozen-thawed in 4 ml and 8 ml volumes. MOT was analysed in fresh semen, as well as immediately after thawing (T0) and 30 min after thawing (T30). INT was analysed using hypoosmotic swelling test (HOS test) in fresh ejaculate (FE), after diluting (DE), and at T0. The differences between FE parameters and frozen-thawed ejaculate parameters, expressing changes that occur during cryopreservation, were calculated. Apart from the effect of thawing protocol, the effect of breed and the effect of quality of FE expressed by MOT immediately after collecting were evaluated, too. Unlike thawing of semen cryopreserved in straws (0,25 and 0,5 ml), thawing using the slow protocol (P1) was the most appropriate for both obser
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/QJ1210109" target="_blank" >QJ1210109: Innovations of bull semen processing and technological procedures of AI dose manufacture leading to improvement of bulls´ inseminating capabilities</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
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Volume of the periodical
63
Issue of the periodical within the volume
10
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
9
Pages from-to
399-407
UT code for WoS article
000445766800003
EID of the result in the Scopus database
2-s2.0-85054718772