SmSP2: A serine protease secreted by the blood fluke pathogen Schistosoma mansoni with anti-hemostatic properties
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A78057" target="_blank" >RIV/60460709:41210/18:78057 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1371/journal.pntd.0006446" target="_blank" >http://dx.doi.org/10.1371/journal.pntd.0006446</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pntd.0006446" target="_blank" >10.1371/journal.pntd.0006446</a>
Alternative languages
Result language
angličtina
Original language name
SmSP2: A serine protease secreted by the blood fluke pathogen Schistosoma mansoni with anti-hemostatic properties
Original language description
SmSP2 comprises three domains, a histidine stretch, TSR1 and a serine protease domain. The cleavage specificity of recombinant SmSP2 was determined using positional scanning and multiplex combinatorial libraries and the determinants of specificity were identified with 3D homology models, demonstrating a trypsin like endopeptidase mode of action. SmSP2 displayed restricted proteolysis on protein substrates. It activated tissue plasminogen activator and plasminogen as key components of the fibrinolytic system, and released the vasoregulatory peptide, kinin, from kininogen. SmSP2 was detected in the surface tegument, esophageal glands and reproductive organs of the adult parasite by immunofluorescence microscopy, and in the excretory secretory products by immunoblotting.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30310 - Parasitology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS Neglected Tropical Diseases
ISSN
1935-2727
e-ISSN
1935-2735
Volume of the periodical
12
Issue of the periodical within the volume
4
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
26
Pages from-to
1-26
UT code for WoS article
000433487700071
EID of the result in the Scopus database
2-s2.0-85046346355