Sample handling and pretreatment as critical points in determining the quality of analytical data during metallothionein determination in wild animals

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F19%3A77282" target="_blank" >RIV/60460709:41210/19:77282 - isvavai.cz</a>
Alternative codes found
RIV/61388955:_____/19:00499050
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1470160X18308641?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1470160X18308641?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ecolind.2018.11.002" target="_blank" >10.1016/j.ecolind.2018.11.002</a>

Result language
angličtina
Original language name
Sample handling and pretreatment as critical points in determining the quality of analytical data during metallothionein determination in wild animals
Original language description
Metallothioneins (MTs) are considered possible markers of environmental pollution, but pre-analysis handling of the tissues in which they are contained can affect their degradation. To determine MT synthesis in wild animals for the purpose of evaluating environmental pollution, the stability of MTs during animal trapping, during the transporting of samples to the laboratory, and during the storing of samples before analysis should be assessed. Five methods of pretreatment processing were tested on 12 male rats under laboratory conditions. MT concentrations were determined by differential pulse voltammetry (DPV) of the modified Brdička reaction. The results show that freeze-drying does not affect the concentration of MTs in the tissue. However, the simulation of extreme field conditions resulted in a significantly lower concentration of MTs in analysed rat liver samples compared with normal conditions. Such samples could be excluded from further evaluation due to differences in the voltammetric record
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10511 - Environmental sciences (social aspects to be 5.7)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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