Identification of suitable reference genes for normalization of quantitative real-time PCR-based gene expression in chicken (Gallus gallus)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F22%3A94070" target="_blank" >RIV/60460709:41320/22:94070 - isvavai.cz</a>

Result on the web

<a href="https://pubmed.ncbi.nlm.nih.gov/35993244/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/35993244/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/age.13252" target="_blank" >10.1111/age.13252</a>

Result language

angličtina

Original language name

Identification of suitable reference genes for normalization of quantitative real-time PCR-based gene expression in chicken (Gallus gallus)

Original language description

The recent availability of genome information greatly facilitates the fundamental research on chicken. In different organs, gene expression patterns can provide clues to understanding the biological functions. For rapid and accurate quantification of gene expression, quantitative real-time PCR (qPCR) has become one of the most widely used methods. However, the success of qPCR data normalization depends on the use of a suitable reference gene and a single reference gene is not universally suitable for all the experiments. Therefore, reference gene validation is a crucial step for different organ tissues of chicken where suitable reference genes for qPCR analysis in varieties of tissues have not been investigated exhaustively so far. In this study, we have selected 30 Gallus gallus candidate reference genes from NCBI, amplified and studied their expression profiles by qPCR in different organ tissues (breast muscle, thigh muscle, heart, liver, spleen, gizzard, and bursa) of chicken. The result showed th

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animal Genetics

ISSN

0268-9146

e-ISSN

—

Volume of the periodical

53

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

881-887

UT code for WoS article

000842491700001

EID of the result in the Scopus database

2-s2.0-85136507383