Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours -- IPO8 and RPL4 are reliable reference genes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33144170" target="_blank" >RIV/61989592:15110/13:33144170 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/1757-2215-6-60" target="_blank" >http://dx.doi.org/10.1186/1757-2215-6-60</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1757-2215-6-60" target="_blank" >10.1186/1757-2215-6-60</a>

Result language

angličtina

Original language name

Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours -- IPO8 and RPL4 are reliable reference genes

Original language description

To ensure a correct interpretation of results obtained with quantitative real-time reverse transcriptionpolymerase chain reaction (RT-qPCR), it is critical to normalize to a reference gene with stable mRNA expression in the tissue of interest. GADPH is widely used as a reference gene in ovarian tumour studies, although lacking tissue-specific stability. The aim of this study was to identify alternative suitable reference genes for RT-qPCR studies on benign, borderline, and malignant ovarian tumours. Weassayed mRNA levels for 13 potential reference genes ? ABL1, ACTB, CDKN1A, GADPH, GUSB, HPRT1, HSP90AB, IPO8, PPIA, RPL30, RPL4, RPLPO, and TBP ?with RT-qPCR in 42 primary ovarian tumours, using commercially pre-designed RT-qPCR probes. Expression stability was subsequently analysed with four different statistical programs (GeNorm, NormFinder, BestKeeper, and the Equivalence test). Expression of IPO8, RPL4, TBP, RPLPO, and ACTB had the least variation in expression across the tumour samp

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Jornal of Ovarian Research

ISSN

1757-2215

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

60-69

UT code for WoS article

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EID of the result in the Scopus database

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