The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F11%3A00166608" target="_blank" >RIV/62156489:43210/11:00166608 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR
Original language description
The selection of reference genes is essential for gene expression studies when using real-time quantitative PCR. Reference gene selection should be performed for each individual experiment because the gene expression level may change in different experimental conditions. In this study, the stability of mRNA expression was determined for seven genes: HPRT1, RPS18, NACA, TBP, TAF4B, RPL32 and OAZ1. The stability of these reference genes was investigated in the skeletal muscle tissue of pig foetuses, piglets and adult pigs using real-time quantitative PCR and SYBR green chemistry. The expression stability of used reference genes was calculated using geNorm application. Different gene expression profiles between the age categories of pigs were found. RPS18has been identified as the gene with the most stable expression in muscle tissue of all pig age categories. HPRT1 and RPL32 were found to have the highest stability in piglets and adult pigs, and in foetuses and adults pigs, respectively
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GA523%2F09%2F0844" target="_blank" >GA523/09/0844: Towards understanding the biology of porcine muscle growth: Comparative gene expression analysis between skeletal muscles of Large White and Wild Boar</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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