Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F08%3A00128656" target="_blank" >RIV/62156489:43210/08:00128656 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig
Original language description
The aim of this study was to develop a set of reliable reference genes for quantification of mRNA expression in the pig. The mRNA expression stability in pig tissues was studied for 4 genes: EEF1A1, GAPDH, HPRT1 and TOP2B. The level of expression was characterized by Ct values for each gene and each tissue. By using the geNorm algorithm, the stability of the reference genes was determined in the diaphragm, heart, kidney, liver, lungs, longissimus muscle, and spleen. On the basis of this information, suitable reference genes can be selected for mRNA expression studies in relevant pig tissues.
Czech name
Ověření referenčních genů pro relativní kvantifikaci genové exprese pomocí reverzně-transkripční PCR v reálném čase u prasete
Czech description
Ověření referenčních genů pro relativní kvantifikaci genové exprese pomocí reverzně-transkripční PCR v reálném čase u prasete
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of applied genetics
ISSN
1234-1983
e-ISSN
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Volume of the periodical
49
Issue of the periodical within the volume
3
Country of publishing house
PL - POLAND
Number of pages
3
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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