A thaumatin-like genomic sequence identification in Vitis vinifera L., Stormy wines and musts based on direct PCR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F18%3A76468" target="_blank" >RIV/60460709:41340/18:76468 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.5219/892" target="_blank" >http://dx.doi.org/10.5219/892</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5219/892" target="_blank" >10.5219/892</a>
Alternative languages
Result language
angličtina
Original language name
A thaumatin-like genomic sequence identification in Vitis vinifera L., Stormy wines and musts based on direct PCR
Original language description
Direct polymerase chain reaction method was use to amplify a thaumatin - like sequence of Vitis vinifera L. in grapes as well as in stormy wines and musts. Thaumatin-like proteins (TLPs) of Vitis vinifera possess beside its function in abiotic and biotic stress response another one - they are able to cause protein haze in wine unless removed prior to bottling. Direct PCR is an approach where omission of DNA extraction is typical prior the amplification of the target site of plant genome. Crude extract or small pieces of plant tissues are used in the analysis directly without steps of extraction and purification of gDNA. The biological material that was used in analysis was collected during August - October 2017 in local stores and winery Sabo and comprises from cultivars Irsai, Muskát, Savignon Blanc, Svatovavrinecké, Dornfelder and Pálava. Direct PCR was performed by a cutted piece of grape tissue and a dilution buffer was use in 1:2 for stormy wine or must, respectively. Direct amplification of tha
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
21101 - Food and beverages
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Potravinárstvo
ISSN
1337-0960
e-ISSN
1337-0960
Volume of the periodical
12
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
226-232
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85043466617