High-proline proteins in experimental hazy white wine produced from partially botrytized grapes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00507851" target="_blank" >RIV/61389030:_____/19:00507851 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/19:73597156
Result on the web
<a href="http://dx.doi.org/10.1002/bab.1736" target="_blank" >http://dx.doi.org/10.1002/bab.1736</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/bab.1736" target="_blank" >10.1002/bab.1736</a>
Alternative languages
Result language
angličtina
Original language name
High-proline proteins in experimental hazy white wine produced from partially botrytized grapes
Original language description
Undesirable effects of the pathogen Botrytis cinerea include reduced quality and quantity of wine grapes. Winemaking is also complicated by the formation of a protein haze in white wines and oxidative browning of red wines. We analyzed proteins in experimental Moravian white wines characterized by their instability and haze formation in bottles during storage despite prior bentonite treatment. To study the relationship of wine proteins and haze, we carried out proteomics on hazy and clear white wines produced with partly or largely botrytized grapes and standard reference wines. Wine proteins were identified after their extraction, electrophoresis, and tryptic digestion by reversed-phase liquid chromatography of peptides, coupled with tandem mass spectrometry. Plant defense proteins, yeast glycoproteins, and various enzymes from Botrytis, particularly hydrolases, were found. As the content of the known haze-active thaumatin-like proteins and chitinases was visually low on stained gels (missing bands) compared to previous studies with unfined wines, other proteins are discussed in terms of the haze formation. As the main novelty, this work reveals the role of high proline-containing proteins in the propensity of white wines to turbidity following prior Botrytis damage of grapes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biotechnology and Applied Biochemistry
ISSN
0885-4513
e-ISSN
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Volume of the periodical
66
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
398-411
UT code for WoS article
000476792600015
EID of the result in the Scopus database
2-s2.0-85061457884