The inhibition of prostaglandin biosynthesis by thymoquinone: cyclooxygenase-1 and -2 in vitro assays

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41610%2F04%3A9838" target="_blank" >RIV/60460709:41610/04:9838 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The inhibition of prostaglandin biosynthesis by thymoquinone: cyclooxygenase-1 and -2 in vitro assays

Original language description

The seeds of Nigella sativa L. (Ranunculaceae) known also as black cummin are widely used in traditional medicine in the Middle East and South Asia for the treatment of various respiratory and gastrointestinal diseases, pain in joints and eczema. Recentstudies have demonstrated its anticancer, anti-inflammatory, antihistaminic, antibacterial and analgesic activities. Many of these activities are attributed to quinone constituents, of which thymoquinone is more abundant compound. We report here resultsof an in vitro inhibition of cyclooxygenase-1 (COX-1) and ?2 (COX-2) activity for by thymoquinone, the main quinone compound of N. sativa seeds. The radiochemical assay was based on the inhibition of prostaglandin E2 and D2 biosynthesis from [14C] radioactive arachidonic acid catalyzed by COX-1 and ?2 respectively. The identification and quantification of the COX reaction metabolites were analyzed by HPLC performed on C18 reversed phase column with an on-line radioactivity flow detector.

Czech name

Inhibice syntézy prostaglandínů prostřednictvím thymochinonu: cyklooxygenasa-1 a -2 testy

Czech description

The seeds of Nigella sativa L. (Ranunculaceae) known also as black cummin are widely used in traditional medicine in the Middle East and South Asia for the treatment of various respiratory and gastrointestinal diseases, pain in joints and eczema. Recentstudies have demonstrated its anticancer, anti-inflammatory, antihistaminic, antibacterial and analgesic activities. Many of these activities are attributed to quinone constituents, of which thymoquinone is more abundant compound. We report here resultsof an in vitro inhibition of cyclooxygenase-1 (COX-1) and ?2 (COX-2) activity for by thymoquinone, the main quinone compound of N. sativa seeds. The radiochemical assay was based on the inhibition of prostaglandin E2 and D2 biosynthesis from [14C] radioactive arachidonic acid catalyzed by COX-1 and ?2 respectively. The identification and quantification of the COX reaction metabolites were analyzed by HPLC performed on C18 reversed phase column with an on-line radioactivity flow detector.

Type

D - Article in proceedings

CEP classification

GM - Food industry

OECD FORD branch

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Project

<a href="/en/project/GA525%2F02%2F0257" target="_blank" >GA525/02/0257: Screening of antibacterial and anti-inflammatory activity of selected species of genus Nigella L.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Sborník abstraktů: International Congress on Natural Product Research

ISBN

nemá

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

1-1

Publisher name

International Congress on Natural Product Research

Place of publication

Phoenix, Arizona, USA

Event location

Phoenix, Arizona, USA

Event date

Jul 31, 2004

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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