A new selective medium for the isolation of glucose non-fermenting bifidobacteria from hen caeca

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A_____%2F00%3A00001245" target="_blank" >RIV/60460709:_____/00:00001245 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/00:00001245

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A new selective medium for the isolation of glucose non-fermenting bifidobacteria from hen caeca

Original language description

Avian caeca contain a large and diverse population of bacteria. Certain genera, including Bifidobacterium, are thought to exert health-promoting effects. Two media were evaluated to determine their sensitivity and selectivity for bifidobacteria in the hen caecal samples: modified Wilkins-Chalgren agar (MW; Oxoid) with the addition of glacial acetic acid (1 ml/l) and mupirocin (100 mg/I) and modified TPY agar (MTPY; ADSA, Spain) with glacial acetic acid (1 ml/l) and mupirocin (100 mg/l). The colonies arising on the plates inoculated with caecal samples were Gram stained, screened for the presence of fructose-6-phosphate phosphoketolase activity, and tested for fermentation patterns using ANAEROtest (Lachema, Czech Republic) and APl 50 CHL (BioMérieux, France) kits. Both agars were selective for bifidobacteria, however, MTPY agar showed higher cfu/g than MW agar. Bifidobacterial counts were higher than 10^10 cfu/g of caecal contents using MTPY agar. Most of strains isolated from this med

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA524%2F99%2F0480" target="_blank" >GA524/99/0480: Enumeration of bifidobacteria an influence of oligosaccharides on biochemical and microbiological characteristics of digestive tract of farm and laboratory anim</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Microbiolocal Methods

ISSN

0167-7012

e-ISSN

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Volume of the periodical

43

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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