The development of a sensitive immunoassay for the detection of new synthetic drugs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F13%3A43903221" target="_blank" >RIV/60461373:22310/13:43903221 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/13:43903221

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The development of a sensitive immunoassay for the detection of new synthetic drugs

Original language description

New synthetic psychoactive drugs are the most destructive and available compounds today. Older drugs as heroin or cocaine destroy person's life but new drugs are more dangerous because these compounds not only cause overdose, a psychotic episode of hallucinations, aggression, impulses and homicidal tendencies. Using of these drugs is widening amongst young people. Consequently, there is growing public interest in development of versatile detection methods enabling to effectively intervene in this field. Traditional methods of analysis of narcotics are gas and liquid chromatography - GC/MS, LC/MS. Their advantages are high sensitivity and reliability. On the contrary, instrumental methods are less rapid and they involve multiple steps in sample preparation and analysis, require expensive equipment and skilled analysts, and therefore they are unsuitable for routine analysis of a large number of samples or on-site determinations. An alternative approach is represented by immunoassays which could be portable and cost-effective, with adequate sensitivity, high selectivity and a simple sample processing. In this work, enzyme -linked imunnosorbent assays have been developed for the detection of selected synthetic cathinones. Carboxylated derivatives of parent drugs were prepared as haptens for synthesis of immunogens and subsequently a series of polyclonal rabbit antibodies were prepared. The antibodies and haptens were used as immunoreagents in competitive Enzyme-linked immunosorbent assay. Under optimal experimental conditions, the most sensitive assay achieved IC50 and limit of detection values of 2 ng/ml and 0.09 ng/mL, respectively. We conclude that after the validation for individual matrices, these methods enable effective fast detection of synthetic cathinones in operational conditions.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

FR - Pharmacology and apothecary chemistry

OECD FORD branch

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Project

<a href="/en/project/VG20122015075" target="_blank" >VG20122015075: New synthetic drugs (NSD) - creation of toxicological database, development and validation of detection methods including fast imunochemical tests, behavioral pharmacology, pharmacokinetics and biotransformation in rats, epidemiology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů