Effect of sterilization methods on electrospun cellulose acetate butyrate nanofibers for SH-SY5Y cultivation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43918743" target="_blank" >RIV/60461373:22310/19:43918743 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/19:43918743 RIV/44555601:13440/19:43894943
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1381514819304833?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1381514819304833?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.reactfunctpolym.2019.104339" target="_blank" >10.1016/j.reactfunctpolym.2019.104339</a>
Alternative languages
Result language
angličtina
Original language name
Effect of sterilization methods on electrospun cellulose acetate butyrate nanofibers for SH-SY5Y cultivation
Original language description
In the biomedical field, the sterilization of nanofibers is of particular importance because it can significantly affect their morphology, physicochemical properties and cytocompatibility. Here, we studied the effect of conventional sterilization with 70% ethanol or ultraviolet (UV) radiation on cellulose acetate butyrate (CAB) nanofibers. Scanning electron microscopy (SEM) revealed that short-term (30 min) UV sterilization had no influence on nanofiber morphology while 70% ethanol led to swelling and, thus, reduced porosity. Water contact angle (WCA) measurements confirmed an increase in wettability after sterilization with ethanol. Fourier transform spectroscopy (FTIR) and electrokinetic analyses indicated only slight changes in the chemical composition Of the CAB nanofibers for both sterilization types. However, longer sterilization time (2 h) led to notable changes in the chemical structure of the scaffolds. In turn, X-ray photoelectron spectroscopy (XPS) revealed changes in the chemical composition, even after short-term sterilization. In combination with FTIR and XPS, differential scanning calorimetry (DSC) identified the hydrolysis of the ethanol-sterilized scaffold and degradation of UV-sterilized one. Human neuroblastoma cell (SH-SY5Y) cultivation on the CAB scaffolds demonstrated significant changes in cell morphology and viability depending on sterilization type. Sterilization of CAB with 70% ethanol was preferable for SH-SY5Y attachment, growth and migration.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20501 - Materials engineering
Result continuities
Project
<a href="/en/project/GA17-00885S" target="_blank" >GA17-00885S: Nanostructured cellulose-based scaffolds with tunable properties for tissue engineering</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Reactive and Functional Polymers
ISSN
1381-5148
e-ISSN
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Volume of the periodical
143
Issue of the periodical within the volume
OCT 2019
Country of publishing house
BE - BELGIUM
Number of pages
8
Pages from-to
"UNSP 104339/1"-8
UT code for WoS article
000489195400003
EID of the result in the Scopus database
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