Honeycomb-patterned poly(L-lactic) acid on plasma-activated FEP as cell culture scaffold

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F20%3A43920909" target="_blank" >RIV/60461373:22310/20:43920909 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/20:43920909 RIV/44555601:13440/20:43895983

Result on the web

<a href="https://doi.org/10.1016/j.polymdegradstab.2020.109370" target="_blank" >https://doi.org/10.1016/j.polymdegradstab.2020.109370</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.polymdegradstab.2020.109370" target="_blank" >10.1016/j.polymdegradstab.2020.109370</a>

Result language

angličtina

Original language name

Honeycomb-patterned poly(L-lactic) acid on plasma-activated FEP as cell culture scaffold

Original language description

The main aim of our work was to design a simple approach for preparation of a specific pattern with a honeycomb-like structure on a solid polymer substrate and to evaluate its cytocompatibility. We have chosen poly-(L-lactic) acid (PLLA) as a source material for formation of the honeycomb-like pattern and perfluorethylenepropylene (FEP) as underlying substrate material. The PLLA pattern formation was induced by improved phase separation with application of a dip-coating technique. Surface morphology, chemistry and wettability of the patterned structure were strongly affected by the initial pretreatment parameters of the FEP substrate, i.e., by the plasma power and exposure time. The pattern formation was unsuccessful without plasma pretreatment. Surface morphology of the PLLA pattern was studied by both of atomic force and scanning electron microscopies. Surface chemistry was analyzed by energy dispersive spectroscopy. The wettability and ability to change electrolyte flow, which might be important for cell-material interaction, were studied by goniometry and zeta-potential, respectively. Due to the morphology and roughness of the prepared pattern, human cells derived from osteosarcoma (U-2 OS) were chosen to evaluate the material cytocompatibility WST-1 assay. Moreover, morphology of cells growing on the honeycomb-like pattern was studied in detail by fluorescence and scanning electron microscopies. We have been able to determine such combination of substrate plasma pre-treatment and honeycomb-like pattern formation on the basis of different amount of target material (PLLA) that led to construction of an excellent scaffold for U-2 OS cell culture.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20501 - Materials engineering

Project

<a href="/en/project/GA19-02495S" target="_blank" >GA19-02495S: Honeycomb like functionalized microporous polymers</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Polymer Degradation and Stability

ISSN

0141-3910

e-ISSN

—

Volume of the periodical

181

Issue of the periodical within the volume

NOV 2020

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

"109370/1"-13

UT code for WoS article

000600681700050

EID of the result in the Scopus database

2-s2.0-85091204398