Structural Basis of the Function of Yariv Reagent - An Important Tool to Study Arabinogalactan Proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F21%3A43923204" target="_blank" >RIV/60461373:22310/21:43923204 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/21:43923204

Result on the web

<a href="http://doi.org/10.3389/fmolb.2021.682858" target="_blank" >http://doi.org/10.3389/fmolb.2021.682858</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmolb.2021.682858" target="_blank" >10.3389/fmolb.2021.682858</a>

Result language

angličtina

Original language name

Structural Basis of the Function of Yariv Reagent - An Important Tool to Study Arabinogalactan Proteins

Original language description

Arabinogalactan proteins are very abundant, heavily glycosylated plant cell wall proteins. They are intensively studied because of their crucial role in plant development as well as their function in plant defence. Research of these biomacromolecules is complicated by the lack of tools for their analysis and characterisation due to their extreme heterogeneity. One of the few available tools for detection, isolation, characterisation, and functional studies of arabinogalactan proteins is Yariv reagents. Yariv reagent is a synthetic aromatic glycoconjugate originally prepared as an antigen for immunization. Later, it was found that this compound can precipitate arabinogalactan proteins, namely, their beta-D-(1→3)-galactan structures. Even though this compound has been intensively used for decades, the structural basis of arabinogalactan protein precipitation by Yariv is not known. Multiple biophysical studies have been published, but none of them attempted to elucidate the three-dimensional structure of the Yariv-galactan complex. Here we use a series of molecular dynamics simulations of systems containing one or multiple molecules of beta-D-galactosyl Yariv reagent with or without oligo beta-D-(1→3)-galactan to predict the structure of the complex. According to our model of Yariv-galactan complexes, Yariv reagent forms stacked oligomers stabilized by π-π and CH/π interactions. These oligomers may contain irregularities. Galactan structures crosslink these Yariv oligomers. The results were compared with studies in literature.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Molecular Biosciences

ISSN

2296-889X

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

682858

Country of publishing house

CH - SWITZERLAND

Number of pages

8

Pages from-to

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UT code for WoS article

000666342300001

EID of the result in the Scopus database

2-s2.0-85108869548