Study of V2CT x -MXene Based Immunosensor for Sensitive Label-Free Impedimetric Detection of SARS-CoV-2 Spike Protein

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F24%3A43931490" target="_blank" >RIV/60461373:22310/24:43931490 - isvavai.cz</a>

Result on the web

<a href="https://pubs.acs.org/doi/full/10.1021/acsami.4c04567" target="_blank" >https://pubs.acs.org/doi/full/10.1021/acsami.4c04567</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsami.4c04567" target="_blank" >10.1021/acsami.4c04567</a>

Result language

angličtina

Original language name

Study of V2CT x -MXene Based Immunosensor for Sensitive Label-Free Impedimetric Detection of SARS-CoV-2 Spike Protein

Original language description

Rapid and reliable immunosensing is undoubtedly one of the priorities in the efficient management and combat against a pandemic, as society has experienced with the SARS-CoV-2 outbreak; simple and cost-effective sensing strategies are at the forefront of these efforts. In this regard, 2D-layered MXenes hold great potential for electrochemical biosensing due to their attractive physicochemical properties. Herein, we present a V2CTx MXene-based sensing layer as an integral part of a label-free immunosensor for sensitive and selective detection of the SARS-CoV-2 spike protein. The sensor was fabricated on a supporting screen-printed carbon electrode using Nafion as an immobilizing agent for MXene and glutaraldehyde, the latter enabling effective binding of protein A for further site-oriented immobilization of anti-SARS-CoV-2 antibodies. A thorough structural analysis of the sensor architecture was carried out, and several key parameters affecting the fabrication and analytical performance of the immunosensor were investigated and optimized. The immunosensor showed excellent electroanalytical performance in combination with an impedimetric approach and exhibited a low detection limit of only 45 fM SARS-CoV-2 spike protein. Its practical applicability was successfully demonstrated by measuring the spike protein in a spiked artificial nasopharyngeal fluid sample.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10402 - Inorganic and nuclear chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ACS Applied Materials &amp; Interfaces

ISSN

1944-8244

e-ISSN

1944-8252

Volume of the periodical

16

Issue of the periodical within the volume

23

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

30196-30208

UT code for WoS article

001236294200001

EID of the result in the Scopus database

2-s2.0-85195071776