Biochemical Detection of Cholinesterase Inhibitors in Water with a New Two-Component Chromogenic Reagent

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F18%3A43915528" target="_blank" >RIV/60461373:22320/18:43915528 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16370/18:43876651

Result on the web

<a href="https://link.springer.com/article/10.1134/S1061934818090101" target="_blank" >https://link.springer.com/article/10.1134/S1061934818090101</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1134/S1061934818090101" target="_blank" >10.1134/S1061934818090101</a>

Result language

angličtina

Original language name

Biochemical Detection of Cholinesterase Inhibitors in Water with a New Two-Component Chromogenic Reagent

Original language description

We propose a simple biochemical detector of cholinesterase inhibitors in water that is based on an enzymatic reaction. The detector was designed as a detection tube containing (i) an indication layer with an immobilized butyrylcholinesterase, (ii) a layer with substrate and chromogenic reagent and (iii) an ampoule with buffer. The chromogenic reagent contained a mixture of two triphenylmethane dyes: Guinea Green B and Fuschsin Basic. The absence of inhibitors was indicated by changing the blue color of the indication layer to violet-red. In the presence of inhibitors, the indication layer remained blue-colored. Color change was evaluated visually (by naked eye). The functionality of the detector was verified on aqueous solutions of alkaloid physostigmine and two chemical warfare agents: sarin and VX. The achieved detection limits were 0.01 μg/mL for physostigmine and sarin and 0.005 μg/mL for VX.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/TA04011060" target="_blank" >TA04011060: Technology for new tube colorimetric biosensor of cholinesterase inhibitors in the air and in liquid samples</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF ANALYTICAL CHEMISTRY

ISSN

1061-9348

e-ISSN

—

Volume of the periodical

73

Issue of the periodical within the volume

9

Country of publishing house

RU - RUSSIAN FEDERATION

Number of pages

6

Pages from-to

900-905

UT code for WoS article

000443826400009

EID of the result in the Scopus database

2-s2.0-85052890296