Enzymatic Determination of Anticholinesterases Using a Composite Carrier
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F16%3A43902414" target="_blank" >RIV/60461373:22320/16:43902414 - isvavai.cz</a>
Alternative codes found
RIV/45808121:_____/16:N0000001 RIV/62157124:16370/16:43874419
Result on the web
<a href="http://dx.doi.org/10.1080/00032719.2016.1151889" target="_blank" >http://dx.doi.org/10.1080/00032719.2016.1151889</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/00032719.2016.1151889" target="_blank" >10.1080/00032719.2016.1151889</a>
Alternative languages
Result language
angličtina
Original language name
Enzymatic Determination of Anticholinesterases Using a Composite Carrier
Original language description
A modified enzyme method is reported for the determination of cholinesterase inhibitors based on the use of a granulated composite carrier with immobilized butyrylcholinesterase. The detection system includes butyrylthiocholine as the substrate and N-(2,3-dimethyl-5-oxo-1-phenyl-3-pyrazoline-4-yl)-2-chloro-5-sulpho-4-iminobenzoquinone as a redox indicator. This method was characterized using carbamate physostigmine and sarin (O-isopropyl methylphosphonofluoridate). The method was also used in a simple tube detector. Evaluation was based on the observation with the naked eye of the decolorization of the redox indicator in comparison to a control time and by tristimulus colorimetry. The limits of detection were 0.5 ?g/mL for physostigmine and 7 x 10?4 mg/m3 for sarin visually, or 0.06 ?g/mL for physostigmine and 1.1 x 10?4 mg/m3 for sarin by colorimetry. ? 2016, Copyright ? Taylor & Francis Group, LLC.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/TA04011060" target="_blank" >TA04011060: Technology for new tube colorimetric biosensor of cholinesterase inhibitors in the air and in liquid samples</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ANALYTICAL LETTERS
ISSN
0003-2719
e-ISSN
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Volume of the periodical
49
Issue of the periodical within the volume
15
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
2418-2426
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-84986296785