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Enzymatic Determination of Anticholinesterases Using a Composite Carrier

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F16%3A43902414" target="_blank" >RIV/60461373:22320/16:43902414 - isvavai.cz</a>

  • Alternative codes found

    RIV/45808121:_____/16:N0000001 RIV/62157124:16370/16:43874419

  • Result on the web

    <a href="http://dx.doi.org/10.1080/00032719.2016.1151889" target="_blank" >http://dx.doi.org/10.1080/00032719.2016.1151889</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1080/00032719.2016.1151889" target="_blank" >10.1080/00032719.2016.1151889</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Enzymatic Determination of Anticholinesterases Using a Composite Carrier

  • Original language description

    A modified enzyme method is reported for the determination of cholinesterase inhibitors based on the use of a granulated composite carrier with immobilized butyrylcholinesterase. The detection system includes butyrylthiocholine as the substrate and N-(2,3-dimethyl-5-oxo-1-phenyl-3-pyrazoline-4-yl)-2-chloro-5-sulpho-4-iminobenzoquinone as a redox indicator. This method was characterized using carbamate physostigmine and sarin (O-isopropyl methylphosphonofluoridate). The method was also used in a simple tube detector. Evaluation was based on the observation with the naked eye of the decolorization of the redox indicator in comparison to a control time and by tristimulus colorimetry. The limits of detection were 0.5 ?g/mL for physostigmine and 7 x 10?4 mg/m3 for sarin visually, or 0.06 ?g/mL for physostigmine and 1.1 x 10?4 mg/m3 for sarin by colorimetry. ? 2016, Copyright ? Taylor & Francis Group, LLC.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/TA04011060" target="_blank" >TA04011060: Technology for new tube colorimetric biosensor of cholinesterase inhibitors in the air and in liquid samples</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    ANALYTICAL LETTERS

  • ISSN

    0003-2719

  • e-ISSN

  • Volume of the periodical

    49

  • Issue of the periodical within the volume

    15

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    9

  • Pages from-to

    2418-2426

  • UT code for WoS article

  • EID of the result in the Scopus database

    2-s2.0-84986296785