Developmnet of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008357" target="_blank" >RIV/60461373:22330/03:00008357 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Developmnet of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples
Original language description
Two enzyme-linked immunosorbent assays (ELISA) with chemiluminescent (CL) detection for the insecticide DDT and the group of DDT-related compounds have been optimized and characterized. Both conjugate-coated ELISAs are based on monoclonal antibodies (MAbs) of different specificity and homologous protein conjugates. Effects of several physicochemical factors (ionic strength, pH, Tween-20 and Bovine serum albumin (BSA) concentrations) and solvents (methanol, ethanol, acetone and N,N'-dimethylformamide) onthe performance of the assays were studied and optimized. For the DDT-selective assay, the sensitivity, estimated as the I-50 value, was 0.6 mug/l, with a linear working range between 0.1 and 2 mug/l and a limit of detection of 0.06 mug/l. For the DDT group-selective assay, the sensitivity was 0.2 mug/l, with a linear working range between 0.07 and 1 mug/l and a limit of detection of 0.04 mug/l. CL-ELISAs were four times more sensitive compared to colorimetric ELISAs. Finally, both immu
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)