The Determination of N-methylcarbamate Pesticides Using Enzyme Immunoassays with Chemiluminescent Detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012904" target="_blank" >RIV/60461373:22330/04:00012904 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The Determination of N-methylcarbamate Pesticides Using Enzyme Immunoassays with Chemiluminescent Detection
Original language description
In the present work, enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb were developed and the analytical parameters of these assays were compared with those of ELISAswith colorimetric detection. The sensitivity of immunochemical methods was expressed as detection limit, linear working range, and I50 value. In comparison with colorimetric ELISA, the ability of the chemiluminescent reagents to detect lower concentrations of HRP allowed to decrease the optimal antibody and conjugate concentrations and to reach better analytical parameters. The experimental comparison of the analytical performance of the ELISAs was carried out by analysing simply diluted fruit juices, spiked at different concentration levels with the above mentioned pesticides. Recovery values for both ELISAs were around 100 % and no matrix effects were observed when fruit juices were diluted 1:20 or more.
Czech name
Stanovení N-methylkarbamátových pesticidů pomocí enzymové imunoanalýzy s chemiluminescenční detekcí
Czech description
Stanovení N-methylkarbamátových pesticidů pomocí enzymové imunoanalýzy s chemiluminescenční detekcí

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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