Immunoprobes for thermally-induced alterations in whey protein structure and their application to the analysis of thermally-treated milks

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012803" target="_blank" >RIV/60461373:22330/04:00012803 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/04:00014771

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Immunoprobes for thermally-induced alterations in whey protein structure and their application to the analysis of thermally-treated milks

Original language description

Polyclonal antisera have been raised to the whey proteins a-lactalbumin [a-La] and b-lactoglobulin [b-Lg], variants A and B. These antibody preparations have been used to develop enzyme-linked immunosorbent assays (ELISAs) for each of these proteins, which had limits of detection of 13 ng/ml [a-La], 27 ng/ml [b-Lg, variant A], and 20 ng/ml [b-Lg, variant B]. The a-La ELISA did not show any cross-reaction with b-Lg, and neither of the b-Lg ELISAs showed a cross-reactivity with a-La. However, despite thealmost identical sequences of variants A and B of b-Lg, the variant A ELISA had a cross-reactivity of 66% with variant B, whilst the variant B ELISA had a cross-reactivity of more than 200% with variant A. The effect of thermal treatment on the immunoreactivity of purified whey proteins was studied by ELISA and related to changes in secondary and tertiary structure determined using CD and fluorescence spectroscopy. The immunoreactivity of a-La determined by ELISA decreased on heating abo

Czech name

Imunosondy pro termálně indukované změny ve struktuře syrovátkových proteinů a jejich aplikace na analýzu tepelně zpracovaných mlék

Czech description

Imunosondy pro termálně indukované změny ve struktuře syrovátkových proteinů a jejich aplikace na analýzu tepelně zpracovaných mlék

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/OK%20384" target="_blank" >OK 384: Rapid, specific detection of Listeria monocytogenes by antibody-based techniques</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Food and Agricultural Immunology

ISSN

0954-0105

e-ISSN

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Volume of the periodical

15

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

77-91

UT code for WoS article

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EID of the result in the Scopus database

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