Isolation and characterization of the Mason-Pfizer monkey virus p12 protein

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012824" target="_blank" >RIV/60461373:22330/04:00012824 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Isolation and characterization of the Mason-Pfizer monkey virus p12 protein
Original language description
The Mason#Pfizer monkey virus (M-PMV) Gag protein, precursor to the structural proteins of the infectious virion, assembles intoimmature capsid-like particles when expressed at high levels in bacterial cells. Similar capsid-like particles can be obtainedby in vitroassembly using a high concentration of isolated Gag. M-PMV Gag contains a p12 protein that has no corresponding analogues in most otherretroviruses and has been suggested to contain an internal scaffold domain (ISD). We have expressed and purified p12 and the N- and Cterminalhalves (Np12 and Cp12) that are predicted to be structurally independent domains. The behavior of these proteins was analyzedusing chemical cross-linking, CD spectroscopy, and electron microscopy. The N-terminal half ofp12 is largely a-helical although the Cterminalportion lacks any apparent ordered structure. Both p12 and Np12 form high-order oligomers in vitro and when expressed in E. coliproduce organized structures that are visible by electron micro
Czech name
Izolace a charakteristika proteinu p12 Mason-Pfizerova opičího viru
Czech description
Izolace a charakteristika proteinu p12 Mason-Pfizerova opičího viru

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
<a href="/en/project/LN00A079" target="_blank" >LN00A079: Center of Integrated Genomics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)