The investigation of the capsid assembly and infectivity of Mason-Prizer Monkey virus frame-shift mutants

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012909" target="_blank" >RIV/60461373:22330/04:00012909 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The investigation of the capsid assembly and infectivity of Mason-Prizer Monkey virus frame-shift mutants
Original language description
Retroviruses regulate the ratios of their polyprotein precursors by frame-shifting events during translation of the genome-length DNA in the host cell. Mason-Pfizer Monkey virus (M-PMV) uses two ribosomal frameshifts in order to synthesize its polyprotein precursors, Pr78 (Gag), Pr95 (Gag-Pro), Pr 180(Gag-Pro-Pol). The nature of polyprotein precursors varies in retroviruses. In order to investigate the requirement for specific polyprotein precursors in assembly, maturation and consequently the virion infectivity, we mutated frame-shifting sites in the M-PMV genome. The resulting viral mutants mimic the polyprotein ratios of HIV or RSV producing only Gag and Gag-Pro-Pol or Gag-Pro and Gag-Pro-Pol polyproteins, respectively. The mutant producing only Gag-Pro-Pol or Gag and Gag-Pro polyproteins were also generated. Properties of these mutants were compared to wild type virus and D26N mutant with inactive proteaseSimultaneous abrogation of both frameshift sites, which results in synthesis
Czech name
Skládání a infektivita frameshiftových mutantů MPVM
Czech description
Skládání a infektivita frameshiftových mutantů MPVM

Project
<a href="/en/project/LN00A079" target="_blank" >LN00A079: Center of Integrated Genomics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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